Publications

• Lu, Y., P.C. Loh, and J.A. Brock. 1989. Isolation, Purification and characterization of infectious hypodermal and hemotopoietic necrosis virus (IHHNV) from penaeid shrimp. J. Virol. Methds. 26:339-344.

• Loh, C. Loh, Y. Lu, and J.A. Brock. 1990. Growth of the penaeid shrimp virus infectious hypodermal and hematopoietic necrosis virus in a fish cell line. J. Virol. Methds. 28:273-280.

• Lu, Y., C.N. Lannan, J.S. Rohovec, and J.L. Fryer. 1990. Fish cell lines establishment and characterization of three new cell lines from grass carp (Ctenopharyngodon idella). In Vitro Cell Dev. Biol. 26:275-279.

• Lu, Y., E.C.B. Nadala, J.A. Brock, and P.C. Loh. 1991. A new virus isolated from infectious hypodermal and hemotopoietic necrosis virus (IHHNV) Infected Penaeid shrimps. J. Virol. Methds. 31:189-196.

• Nadala, E.C.B., Y. Lu, P.C. Loh, and J.A. Brock. 1992. Infection of Penaeus stylirostris (Boone) with a rhabdovirus isolated from Penaeus spp. Fish pathol. 27(3):143-147.

• Nadala, E.C.B., Y. Lu, P.C. Loh, and J.A. Brock 1992. A streptavidin-biotin-enhanced nitrocellulose enzyme immunoassay for the detection of rhabdovirus of penaeid shrimps from infected animals. J. Virol. Methds. 39:227-229.

• Lu, Y. and P. C. Loh. 1992. Some biological properties of a rhabdovirus isolated from penaeid shrimp. Arch Virol. 127:339-343.

• Nadala, E.C.B., Y. Lu, and P.C. Loh. 1993. Primary culture of lymphoid, nerve, and ovary cells from Penaeus stylirostris and Penaeus vannamei. In Vitro Cell Dev. Biol. 29A:620-622.

• Lu, Y. and P.C. Loh. 1994. Viral structural proteins and genome analyses of rhabdovirus of penaeid shrimp (RPS). Dis. Aquat. Org. 19:187-192.

• Lu, Y. and P. C. Loh. 1994. Serological studies of the rhabdovirus of penaeid shrimp (RPS) and its relationship to three other fish rhabdoviruses. J. Fish Dis. 17: 303-309.

• Lu, Y., L.M. Tapay, J.A. Brock, and P.C. Loh. 1994. Infection of the yellow head baculo-like virus (YBV) in two species of penaeid shrimp, Penaeus stylirostris (Stimpson) and Penaeus vannamei (Boone). J. Fish Dis. 17:649-656.

• Lu, Y. and P.C. Loh. 1994. Infectivity studies of rhabdovirus in penaeid blue shrimp. Aquaculture International. 2:123-127.

• Lu, Y., L.M. Tapay, P.C. Loh, J.A. Brock, and R.B. Gose. 1995. Distribution of yellow head virus (YHV) in selected tissues and organs of penaeid shrimp, Penaeus stylirostris. Dis. Aquat. Org. 23:67-70.

• Lu, Y., L.M. Tapay, P.C. Loh, J.A. Brock, and R.B. Gose. 1995. Development of a quantal assay in primary shrimp cell culture for yellow head virus (YHV) of penaeid shrimp. J. Virol. Methds. 52:231-236.

• Tapay, L.M., Y. Lu, J.A. Brock, E.C.B. Nadala, and P.C. Loh. 1995. Transformation of primary cultures of lymphoid ( Oka) organ from a penaeid shrimp, Penaeus stylirostris with simian virus-40 T-antigen. Proc. Exp. Biol. Med. 209:73-78.

• Lu, Y., L.M. Tapay, and P.C. Loh. 1996. Development of a nitrocellulose-enzyme immunoassay (NC-EIA) for the detection of yellow head virus (YHV). J. Fish Dis. 19:9-13.

• Tapay, L.M., Y. Lu, R.B. Gose, E.C. Jr. Nadala, J.A. Brock, and P.C. Loh. 1997. Development of an in vitro quantal assay in primary cell cultures for a non-occluded baculo-like virus of penaeid shrimp. J. Virol. Methds. 64:37-41.

• Loh, P.C., L.M. Tapay, and Y. Lu. 1997. Quantal assay of shrimp viruses in primary shrimp lymphoid cell cultures. In: K. Maramorosch and J. Mitsuhashi (editors), Invertebrate Cell Culture. Science Publishers, Inc. U.S.A. pp. 253-259.

• Loh, P.C., L.M. Tapay, Y. Lu, and E.C.B. Nadala. 1997. Viral pathogens of the penaeid shrimp. Advances in Virus Research. 48:1997.

• Tapay, L.M., Y. Lu, R.B. Gose, J.A. Brock, and P.C. Loh. 1997. Infection of white-spot baculovirus (WSBV) in two species of penaeid shrimp Penaeus stylirostris (Stimpson) and P. vannamei (Boone). Diseases in Asian Aquaculture III 3:297-302.

• Lu, Y., L.M. Tapay, P.C. Loh, J.A. Brock, and R.B. Gose. 1997. Chinese baculo-like virus (CBV), a highly pathogenic viral agent found in cultured penaeid shrimp. Aquaculture International 5:277-282.

• Lu, Y., V. Planelles, X. Li, C. Palaniappan, B. Day, P. Challita-Eid, R. Amado, D. Stephens, D.B. Kohn, A. Bakker, P. Fay, R. A. Bambara, and J.D. Rosenblatt. 1997. Inhibition of HIV-1 replication using a mutated tRNA lys-3 primer. J. Biol. Chem. 272(23): 14523-14531.

• Gonzalez, S., G. Tirado, G. Revuelta, Y. Yamamura, Y. Lu, V.R. Nerurkar, and R. Yanagihara. 1998. CCR5 chemokine receptor genotype frequencies among Puerto Rican HIV-1-seropositive individuals. Bol. Assoc. Med. P. R. 90:12-15.

• Loh, P.C., L.M. Tapay, C.B. Nadala, and Y. Lu. 1998. A SDS/PAGE/Western blot/EIA protocol for the specific detection of shrimp viral pathogens. In: Gal and Halvorson (eds) New Developments in Marine Biotechnology. Plenum Press, New York. pp.295-302.

• Lu, Y., A.A. Aguirre, R.C. Braun, and P.C. Loh. 1998. Establish. of monk seal cell lines. In Vitro Cell Dev. Biol. 34:367-369.

• Lu, Y., V.R. Nerurkar, A.A. Aguirre, T.M. Work, G.H. Balazs, and R. Yanagihara. 1999. Establishment and characterization of 13 cell lines from a green sea turtle (Chelonia mydas) with fibropapillomas. In Vitro Cell Dev. Biol. 35(7):389-393.

• Lu, Y., V.R. Nerurkar, W.M. Dashwood, C.L. Woodward, S. Ablan, C.M. Shikuma, A.Grandinetti, H. Chang, H.T. Nguyen, Z. Wu, Y. Yamamura, W.O. Boto, A. Merriwether, T. Kurata, R. Detels, and R. Yanagihara. 1999. Genotype and allele frequency of a 32-base pair deletion mutation in the CCR5 gene in various ethnic groups: absence of mutation among Asians and pacific islanders. International Journal of Infectious Diseases. 3(4):186-191.

• Lu, Y, V.R. Nerurkar, A.A. Aguirre, T.M. Work, G.H. Balazs, and R. Yanagihara. 2000. Identification of a small naked virus associated with green sea turtle fibropapilloma. J. Virol. Methds. 86:25-33.

• Lu, Y., Q. Yu, J.P. Zamzow, Y. Wang, G.S. Losey, G.H. Balazs, V.R. Nerurkar, and R. Yanagihara. 2000. Detection of green turtle herpesviral sequence in saddleback wrasse: A possible mode of transmission of green turtle fibropapilloma. J. Aquatic Animal health 12:58-63.

• Lu, Y., Y. Wang, Q. Yu, A.A. Aguirre, G.H. Balazs, V.R. Nerurkar, and R. Yanagihara. 2000. Detection of herpesviral sequences in tissues of green turtles with fibropapilloma using polymerase chain reaction. Arch. Virol. 145:1885-1893.

• Yu, Q., Y. Lu, V.R. Nerurkar, and R. Yanagihara. 2000. Turtle tumor susceptibility gene TSG101: Full-length cDNA sequence, genomic architecture, and absence of abnormal transcript in GTFP. J. Aquatic Animal Health 12:274-282.

• Yu, Q., Y. Lu, V.R. Nerurkar, and R. Yanagihara. 2000. Amplification and analysis of DNA flanking known sequences of a novel herpesvirus from green turtles with fibropapilloma. Archives of Virology 145:2669-2676.

• Yu, Q., Y. Lu, V.R. Nerurkar, and R. Yanagihara. 2001. Rapid acquisition of entire DNA polymerase gene of a novel herpesvirus from green turtles fibropapilloma by a genomic walking technique. J. Virol. Methds. 91:183-195.

• Zheng, X., C. Johnson, Y. Lu, R. Yanagihara, S. Hollingshead, M. Crain, W. Benjamin, and K.B. Waites. 2001. Clinical isolates of Streptococcus pneumoniae resistant to levofloxacin contain mutations in both gyrA and parC genes. Antimicrobial agents. 18:373-378.

• Lu, Y., A.A. Aguirre, C. Hamm, Y. Wang, Q. Yu, P.C. Loh, and R. Yanagihara. 2001. Establishment, cryopreservation, and growth of 11 cell lines prepared from a juvenile Hawaiian monk seal, Monachus schauinslandi. Methods In Cell Science 22:115-124.

• Lu, Y., L. He, D. Brock, J. Brock, and R. Bullis. 2001. Establishment and growth of cell lines from aquacultured fish in Hawaii for diagnosis and isolation of pathogenic viruses. CTSA Regional Notes 12:4-7.

• Yu, Q., B. Shiramizu, V.R. Nerurkar, N. Hu, C.M. Shikuma, M.E. Melish, K. Cascio, A. Imrie, Y. Lu, and R Yanagihara. 2002. TT virus: preferential distribution in CD19+ peripheral blood mononuclear cells and lack of viral integration. J. Medical Virology. 66:276-284.

• Lu, Y., Y. Wang, Q. Yu, V.R. Nerurkar, and R. Yanagihara. 2003. RT-PCR detection of gene expression of a novel reptilian herpesvirus in tumor tissues of green turtles with fibropapilloma. Arch. Virol. 148:1155-1163.

• Lu, Y., C. Liu, L. Zeng, Z. Lin, S. Dewhurst , S. Gartner, and V. Planelles. 2003. Efficient gene transfer into human monocyte-derived macrophages using defective lentiviral vectors. Cellular and Molecular Biology. 49:1151-1156.

• Lu, Y., A.A. Aguirre, Y. Wang, P.C. Loh, and R. Yanagihara. 2003. Viral susceptibility of newly established cell lines from the Hawaiian monk seal. Dis. Aquat. Org. 57:183-191.

• Nigro, O., Q. Yu, V. R. Nerurkar, R. Yanagihara, and Y. Lu. 2003. Sequence and characterization of a novel DNA binding protein gene of a reptilian herpesvirus associated with green turtle fibropapilloma. Arch Virol 149:337-347.

• Nigro, O., A.A. Aguirre, and Y. Lu. 2004. Nucleotide sequence and transcriptional analysis of an ICP18.5 assembly protein (UL28) gene of a green turtle herpesvirus pathogenically associated with green turtle fibropapilloma. J. Virol. Methds. 120:107-112.

• Zhao, Z.S., D. Montgomery-Brock, C.S. Lee, and Y. Lu. 2004. Establishment, characterization and viral susceptibility of 3 new cell lines from snakehead, Channa striatus (Blooch) Methds. Cell Sci. 2:155-166.

• Wang, G., S. LaPatra, L. Zeng, Z. Zhao, and Y. Lu. 2004. Establishment, growth, cryopreservation and species of origin identification of three cell lines from white sturgeon, Acipenser transmontanus. Methds. Cell Sci. 25:211-220.

• Wu, C.X. and Y. Lu. 2005. Addition of high molecular weight dextran in calcium phosphate-mediated transfection significantly improves gene transfer efficiency. Cellular and Molecular Biology (in press).

• Zeng, L.B., V. Planelles, Ziye Sui, S. Gartner, S.B. Maggirwar, S. Dewhurst, L.B. Ye, V.R. Nerurkar, R. Yanagihara, and Y. Lu. 2005. Human immunodeficiency virus type 1 (HIV-1) based defective lentiviral vectors efficiently transduce human monocyte-derived macrophages and suppress replication of wild-type HIV-1. J. Gene Med. (in press)

• Lu, Y. and P. Sun. 2005. Viral resistance in shrimp that express an antisense Taura syndrome virus coat protein gene. Antiviral Research (in press).

• Zhao, Z.S. and Y. Lu. 2005. Characterization and viral susceptibility of two newly established cell lines from bluefin trevally Caranx melampygus (Cuvier). Dis. Aquat. Org. (submitted).

• Zeng, L.B., V. V.R. Nerurkar, L.B. Ye, R. Yanagihara, and Y. Lu. 2005.Efficient production of human immunodeficiency virus type 1 (HIV-1)-based vectors for gene transduction of in vitro cell cultures J. Virol. Methds. (submitted).

• Luo, H.Y., J.M. Wu, S.M. Yang, V. Nerurkar, R. Yanagihara, and Y. Lu. 2005. Effective elevation of monocyte transmigration into the CNS by chemical opening of the BBB. (submitted).

• Zeng, L.B., S.M. Yang and Y. Lu. 2005. Comparative studies on different viral vectors for their gene transduction of primary cultures of mouse monocytes in vitro. J. Med. Virol. (submitted).

S11 NS043499
NINDS, NIH
Role: Principal Investigator
Title: Development of Gene Transfer Approaches for NeuroAIDS

.

9/1/02-8/31/07

Pilot Project, G12 RR003061
NCRR, NIH
Role: Co-Investigator; Activity Leader: V.R. Nerurkar; Program Director: R. Yanagihara
Title: Mutant tRNA-Mediated Anti-HIV-1 Infection: A Novel Gene Therapy Approach.

Aquaculture Pilot Project
Center for Tropic and Subtropic Aquaculture
Role: Principal Investigator
Title: Virology Health Services for Hawaiian Aquaculture.

12/1/99-7/31/05

UH-OTTED
Role: Principal Investigator
Title: Molecular Cloning and Identification of Shrimp b -actin Gene.

6/1/05-5/31/06

Shrimp Project
Genentex LLC
Role: Principal Investigator
Title: Production and Challenge of Virus-Resistant Strains of Shrimp.

9/1/05–8/31/08

0000
Role: Principal Investigator
Title: Establishment of in vitro Cell Culture Model for siRNA-based Protection from West Nile Virus Infection.